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. 2003 Jan;131(1):309–316. doi: 10.1104/pp.011171

Figure 2.

Figure 2

Elution profile of BE1-derived SUV. SUV composed of BE1 phospholipids were prepared with 100 mm CF in 20 mm Tris-HCl (pH 7.4), then separated by Superose 6 gel filtration. The fluorescence of CF of each fraction was measured before (white circles) and after (black circles) addition of 2% (w/v) Triton X-100. The concentration of phospholipid was also determined (dashed line). The peak centered on fraction 8 corresponds to multilamellar vesicle (MLV). The peak centered on fraction 12 corresponds to SUV. The left ordinate indicates CF fluorescence (counts per second × 10−6) and right ordinate indicates the concentration of phospholipid.