Table II.

Authentication of GA biosynthetic intermediates and additional products in yeast extracts

Reference or Putative Compounds	KRI	Characteristic Ions– Relative Abundance as % of Base Peak
		404(M+)	389	314	299	255	254	239	223	199
ent-7α-hydroxy KA METMS standard	2,459	22	8	100	32	69	59	43	19	22
ent-7α-hydroxy KA METMS	2,460	35	13	100	33	67	60	45	19	23
		330(M+)	287	270	255	242	241	239	227	199
GA12 aldehyde ME standard	2,437	12	14	40	14	24	100	24	14	10
GA12 aldehyde ME	2,436	11	14	40	15	25	100	24	15	11
		360(M+)	328	300	285	241	240	225	185
GA12 ME standard	2,415	1	17	100	24	35	29	20	12
GA12 ME	2,416	1	17	100	22	35	31	26	15
		388(M+)	345	298	283	270	255	227	163	137
7β-Hydroxykaurenolide TMS standard	2,557	2	9	100	15	17	12	10	12	58
7β-Hydroxykaurenolide TMS	2,552	1	10	100	17	22	14	13	27	95
		492(M+)	477	402	387	343	327	269	253	209
ent-6α,7α-dihydroxy KA METMSa	2,593	<1	92	16	5	4	8	100	16	50

GC-MS relative ion abundances and Kovats' retention index (KRI) were compared with authentic standards. Samples are methyl ester (ME), trimethyl silyl (TMS), or methyl ester trimethyl silyl (METMS) derivatives.

^aTentative identification based on comparison with published spectra (Gaskin and MacMillan, 1991).