Table II.
Amino Acid | Total Amino Acid Concentration
|
15N Enrichment
|
Position of Mono-15N Label
|
Total 15N Concentrationa
|
||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Meanb | se | Mean | se | n | Mean | se | n | Mean | ||||
μmol g−1 fresh wt | atom% excess | % | μmol g−1 fresh wt | |||||||||
Asn | 16.91 | 1.65 | Mono-15N | 8.17 | 0.27 | 11 | Amino | 73 | 6 | 6 | Amino | 1.33 |
Amide | 27 | 6 | 6 | Amide | 0.70 | |||||||
Double 15N | 1.91 | 0.12 | 12 | |||||||||
GABA | 5.72 | 0.67 | 33.39 | 2 | 1.91 | |||||||
Gln | 1.55 | 0.20 | Mono-15N | 15.74 | 2.08 | 5 | Amino | 98 | 2 | 3 | Amino | 0.34 |
Amide | 2 | 2 | 3 | Amide | 0.11 | |||||||
Double 15N | 6.59 | 1.10 | 4 | |||||||||
Ala | 1.24 | 0.12 | 25.02 | 0.80 | 7 | 0.31 | ||||||
Asp | 0.76 | 0.05 | 14.22 | 1.27 | 10 | 0.11 | ||||||
Glu | 0.35 | 0.04 | 27.86 | 0.98 | 5 | 0.10 |
The values given for amino and amide group total 15N concentration include both mono- and double-labeled molecules.
n = 3.