Table I.
cDNA libraries
| Library | Conditions | Strain | Normalization | Project No. | Clones |
|---|---|---|---|---|---|
| Core | TAP light, TAP dark, HS + CO2, HS | 21gr | Not Normalized | 874 | 768 |
| Core | As above | 21gr | Normalized | 894 | 10,080 |
| Core | As above | 21gr | Subtracted (894) | 1,024 | 12,096 |
| Stress I | NO3 to NH4 (30 min, 1, 4 h), NH4 to NO3 (30 min, 1, 4 h), TAP-N (30 min, 1, 4 h), TAP-S (30 min, 1, 4 h), TAP-P (4, 12, 24 h) | 21gr | Normalized | 963 | 12,000 |
| Stress II | NH4 to NO3 (24 h), H2 production (0, 12, 24 h), TAP + H2O2 (1, 12, 24 h), TAP + Sorbitol (1, 2, 6, 24 h), TAP + Cd (1, 2, 6, 24 h) | 21gr | Normalized | 1,031 | 10,752 |
| Stress III | TAP-Fe, TAP-Cu, TAP-O2, TAP high light, HS high light (0, 0.5, 1, 2, 4, 6, 12 h) | 21gr | Normalized | 3,510 | Ongoing |
| Deflagellation | 15, 30, 60 min | 21gr | Normalized | 1,030 | 12,480 |
| Gametogenesis-Zygote | Gamete (2, 8, 10, 12, 15, 17 h), Zygote (30, 60 min) | 21gr | Normalized | 3,511 | Ongoing |
| S1D2 | S1D2 | Normalized | 925 | 124 |
Conditions of cell growth before RNA preparation, project number, and the number of clones sequenced are presented. -N, -S and -P refer to medium devoid of a nitrogen, sulfur, and phosphorus source, respectively. The normalization procedure is described in the text.