Table II.
Identification of carrier proteins from MS/MS data
| Spot No. | Gel MM | MS/MS Spectra | Match to At | Match Carrier | Annotation of Carrier | Chr Locus | MM | GRAVY |
|---|---|---|---|---|---|---|---|---|
| 1 | 38,000 | 15 | 9 | 4 | Putative carrier protein | At4g01100 | 38325 | 0.004 |
| 2 | 37,000 | 6 | 4 | 1 | Putative carrier protein | At4g01100 | 38325 | 0.004 |
| 3 | 36,000 | 8 | 1 | 0 | ||||
| 4 | 35,000 | 13 | 7 | 0 | ||||
| 5 | 34,000 | 20 | 16 | 7 | PiC | At5g14040 | 40089 | 0.145 |
| 6 | 33,000 | 18 | 10 | 0 | ||||
| 7 | 32,000 | 14 | 10 | 0 | ||||
| 8 | 31,000 | 37 | 22 | 8 | ANT | At3g08580 | 41475 | −0.121 |
| 3 | ANT | At5g13490 | 41746 | −0.111 | ||||
| 4 | DTC | At5g19760 | 31912 | 0.129 | ||||
| 9 | 30,000 | 45 | 35 | 5 | UCP | At3g54110 | 32662 | 0.176 |
| 10 | 28,000 | 13 | 4 | 0 |
A total of 189 MS/MS spectra derived from 10 protein bands representing 28- to 38-kD mitochondrial membrane proteins were matched to the Arabidopsis database using Mascot or derived amino acid sequence compared via near exact match BLAST at NCBI. Total spectra derived from each band (MS/MS spectra), the no. successfully matched to an Arabidopsis gene (Match to At), and the no. matched to carrier family proteins (Match carrier) are indicated. The predicted molecular mass (MM) and hydrophobicity (GRAVY) of each identified carrier are noted.