Figure 2.

Analyses of the NtC7. A, The nucleic acids are presented on the top line and the derived one-letter amino acid sequence is shown below. The stop codon is indicated by an asterisk. The original NtC7 clone obtained by FDD screening is underlined. The position of cleaving site of the predicted signal peptide is indicated by a black triangle. The predicted transmembrane region is indicted by small letters. Leu and Ile near the transmembrane domain are marked with circles. The accession number of the cDNA is AB087235 under the name of NtC7. B, Northern-blot hybridization. Total RNA was probed with the full-length NtC7 cDNA, and transcript size was estimated from the migration position of marker RNAs (M) stained with ethidium bromide (EtBr) and signals because of cross hybridization. C, Southern-blot analysis. Genomic DNA (20 μg per lane) was digested with the indicated restriction endonuclease, separated on an agarose gel, blotted, and probed with a radioactively labeled NtC7 fragment.