Figure 5.

Effects of osmotic and salt stresses on NtC7 transcript accumulation. Healthy leaves were wounded by cutting with a pair of scissors, and wounded (local) and unwounded adjacent (systemic) leaves were sampled at the indicated time points, and RNAs assayed by northern hybridization with the indicated probes (A). Healthy leaves were detached and put in buffer solution. At 4 h after the first wounding, leaves were transferred to a buffer solution with or without 500 mm mannitol (B) or 200 mm NaCl (C) for the indicated time period, and RNAs were assayed by northern hybridization with the indicated probes.