Table II.
Seed phytic acid and inorganic phosphate in the ZmIpk Mu-insertion mutants
| F2 Seeds | PCR | Pi | PAP | Pi Mean | PAP Mean | Pi Increase | PAP Reduction |
|---|---|---|---|---|---|---|---|
| 1.3-kb fragment | mg g−1 | -fold | % | ||||
| ZmIpk-mum1−01 | + | 0.33 | 2.44 | ||||
| ZmIpk-mum1−02 | + | 0.12 | 2.44 | ||||
| ZmIpk-mum1−05 | + | 0.14 | 2.46 | ||||
| ZmIpk-mum1−06 | + | 0.26 | 2.37 | ||||
| ZmIpk-mum1−07 | + | 0.21 | 2.28 | ||||
| ZmIpk-mum1−08 | + | 0.24 | 2.62 | 0.22 | 2.44 | ||
| ZmIpk-mum1−03 | − | 0.90 | 1.56 | ||||
| ZmIpk-mum1−04 | − | 0.65 | 1.72 | ||||
| ZmIpk-mum1−09 | − | 0.89 | 1.94 | ||||
| ZmIpk-mum1−10 | − | 0.86 | 1.80 | 0.82 | 1.75 | 2.8 | 28 |
| ZmIpk-mum2−01 | + | 0.10 | 3.15 | ||||
| ZmIpk-mum2−02 | + | 0.14 | 2.54 | ||||
| ZmIpk-mum2−04 | + | 0.10 | 2.82 | ||||
| ZmIpk-mum2−05 | + | 0.10 | 2.62 | ||||
| ZmIpk-mum2−07 | + | 0.16 | 2.69 | ||||
| ZmIpk-mum2−08 | + | 0.11 | 2.41 | ||||
| ZmIpk-mum2−09 | + | 0.16 | 2.73 | ||||
| ZmIpk-mum2−10 | + | 0.07 | 3.16 | 0.12 | 2.76 | ||
| ZmIpk-mum2−03 | − | 0.66 | 2.06 | ||||
| ZmIpk-mum2−06 | − | 0.69 | 1.78 | 0.67 | 1.92 | 4.7 | 30 |
| ZmIpk-mum3−01 | + | 0.17 | 2.29 | ||||
| ZmIpk-mum3−02 | + | 0.12 | 2.15 | ||||
| ZmIpk-mum3−03 | + | 0.19 | 2.21 | ||||
| ZmIpk-mum3−04 | + | 0.18 | 2.11 | ||||
| ZmIpk-mum3−07 | + | 0.21 | 1.95 | ||||
| ZmIpk-mum3−08 | + | 0.15 | 2.02 | ||||
| ZmIpk-mum3−09 | + | 0.12 | 2.12 | ||||
| ZmIpk-mum3−10 | + | 0.14 | 2.21 | 0.16 | 2.13 | ||
| ZmIpk-mum3−05 | − | 0.51 | 1.53 | ||||
| ZmIpk-mum3−06 | − | 0.52 | 1.45 | 0.52 | 1.49 | 2.2 | 30 |
Individual F2 mature seeds from an ear were analyzed for inorganic phosphate and phytic acid. The Pi and phytic acid are expressed as Pi P and phytic acid P (PAP), respectively. PCR was conducted with primers flanking the ZmIpk open reading frame, and a product of 1.3-kb fragment is expected for intact ZmIpk.