Table I.

Trehalose contents and trehalase activities in monocot and dicot transgenic plants

Transgenic Plants	Transgene	Trehalose Production	Trehalase Activitya	References
		mg g fresh wt−1	μmol h mg protein−1
Rice	E. coli otsA/otsB fusion	0.31–1.076	0.174 ± 0.05	This study
Tobacco (Nicotiana tabacum)	Yeast TPS1	0.16–0.64 (0.8–3.2)b	NTc	Holmström et al. (1996)
Tobacco	Yeast TPS1	0.17	NT	Romero et al. (1997)
Tobacco	E. coli otsA and otsB	0.038–0.04 (0.19–0.20)b	NT	Pilon-Smits et al. (1998)
Tobacco	E. coli otsA and otsB	0.005–0.07	0.442–0.679	Goddijn et al. (1997)
Tobacco	E. coli otsA	0.02–0.11	0.442–0.679	Goddijn et al. (1997)
Potato (Solanum tuberosum)	E. coli otsA and otsB	0.003–0.02	0.048 ± 0.006 (0.125 ± 0.003)d	Goddijn et al. (1997)

Leaf tissues were ground in liquid nitrogen and extracted at 100°C with water. After the extract was clarified, trehalose was quantitated by HPIC with a Carbo-Pak PA1 column using the DX500 HPIC system (Dionex 500). Trehalase activity was measured by estimating both the glucose produced by hydrolysis of trehalose and trehalose reduced.

^aTested in leaf tissues. 

^bNos. in parentheses refer to milligram per gram dry wt, as described in the literature. 

^cNT, Not tested. 

^dTested in tuber.