Figure 1.
Targeting of the kinin B1-receptor gene. (A) Schematic representation of the targeting strategy with the wild-type B1 locus, the recombination vector, and the predicted structure of the targeted gene. The probe used for Southern blot and the length of the EcoRI restriction fragments are indicated. (B) Southern blot analysis of tail DNA from offspring of a cross between two heterozygous B1-deficient mice digested with EcoRI and hybridized with the aforementioned probe. (C) RNase protection assay detecting B1, IL-1β, and β-actin mRNA in untreated animals or 5 h after injection of 10 μg/kg LPS. (D) Isometric responses of isolated ileum from wild-type (WT) and B1-deficient mice (KO) to BK and des-Arg9-BK (1 μM each). Only the B2 receptor-dependent contractile response to BK is preserved in B1-deficient mice.