Figure 2.

Expression pattern of Cu/ZNSOD [SOD1.1] (A) and CA transcripts (B). Wheat total RNA was isolated from shoots of wheat cv BDME, cv Dagdas, and cv Kirgiz grown in low-Zn (0.1 pm) or sufficient Zn (150 pm) medium. The northern blot was equally loaded with 15 μg of total RNA per lane. C, Ethidium bromide-stained rRNA is shown as a loading control. Filters were hybridized with radiolabeled ([α-³²P]dCTP) SOD1.1 or CA probes overnight, washed under high-stringency conditions, and exposed to x-ray film, as described in “Materials and Methods.” Similar results were obtained in three independent experiments.