Figure 3.

Expression analysis of SOD1.1 (A) and CA (B) transcripts. Wheat poly(A⁺) mRNA was directly isolated from shoots of wheat cv BDME, cv Dagdas, and cv Kirgiz grown in low Zn (0.1 pm) or high Zn (1 μm) medium. Equal amounts of mRNA (2.5 μg) were loaded per lane. C, Ethidium bromide-stained rRNA band included to show RNA loading. Filters were hybridized with radiolabeled ([α-³²P]dCTP) SOD1.1 or CA probes overnight, washed under high-stringency conditions, and exposed to x-ray film, as described in “Materials and Methods.” Each experiment was repeated at least three times with similar results.