Figure 3.

Tocopherol composition and levels in seed of wild-type and homozygous 35S::HPT1 Arabidopsis plants. Seed was extracted and analyzed for tocopherols by normal phase HPLC. Tocopherol levels are expressed as an average ± sd of three analyses per each line in a representative experiment. Total tocopherol levels in picomoles per milligram of seed are indicated above the error bar of each line. Arabidopsis seeds accumulate predominantly γ-tocopherol, which increased up to 40% in 35S::HPT1 compared with wild type. The levels of α-tocopherol did not change significantly, whereas δ-tocopherol increased up to 2-fold in some transgenic lines. β-Tocopherol was not detected. With the exception of 35S::HPT1-16, statistical significance for total tocopherol levels of all transgenic lines relative to wild type was P < 0.001.