Tocopherol composition and levels in seed of wild-type, 35S::γ-TMT, 35S::HPT1, and 35S::γ-TMT/35S::HPT1 overexpressers. A, α-, β-, γ-, and δ- tocopherols; B, β- and δ-tocopherols. Tocopherol analysis of mature dry seed was performed as described in “Materials and Methods.” Each line is presented as an average of three independent measurements of three replicates. Total tocopherol levels of 35S::HPT1 and 35S::γ-TMT/35S::HPT1 lines were significantly higher than wild type (P < 0.05). The majority of γ- and δ-tocopherols was converted to α- and β-tocopherols in 35S::γ-TMT/35S::HPT1 overexpressers.