Table III.
Total chlorophyll, carotenoids, and PQ-9 levels in wild-type and 35S::HPT1 Arabidopsis leaves and seeds
| Line | Leaf Content
|
Seed Content
|
|||
|---|---|---|---|---|---|
| Chlorophylls | Carotenoids | PQ-9 | Chlorophylls and PQ-9 | Carotenoids | |
| nmol mg−1 | pmol mg−1 | pmol mg−1 | |||
| Wild type | 1.00 ± 0.06 | 0.43 ± 0.04 | 82 ± 8 | nd | 30.3 ± 0.2 |
| 35S::HPT1-11 | 1.01 ± 0.10 | 0.45 ± 0.04 | 80 ± 5 | nd | 28.3 ± 0.6 |
| 35S::HPT1-54 | 0.98 ± 0.08 | 0.43 ± 0.05 | 83 ± 4 | nd | 35.9 ± 0.2 |
In leaves, chlorophyll and carotenoid levels were determined in 90% (v/v) methanol as described previously (Lichtenthaler, 1987). Twenty milligrams of seed was extracted, and pigments were analyzed by reverse-phase HPLC. Lutein made up >95% of total seed carotenoids (data not shown), and its levels were taken as total carotenoid levels. Chlorophylls and PQ-9 were not detected (nd) in dry Arabidopsis seed. Chlorophyll and carotenoid levels in 35S::HPT1 leaves and seeds were comparable with wild-type levels. Pigment levels are an average ± sd of three measurements.