Table II.

The HPLC gradient used to separate amino acids

Time	Solvent B
min	%
0	15
6.2	24
8.5	25
9.6	27
20	40
25	51
27.5	52
30	52
32	70
34	70
36	15

Solvent A was 65 mm sodium acetate and 7% (v/v) DMF in water at pH 6.5. Solvent B was acetonitrile.