Figure 3.

Relationship between Bostwick values, vis1 transcript accumulation, and DNA polymorphism for the vis1-hta and vis1-lta alleles. A, Bostwick values were from the microwave oven-processed tomato juice of fruits grown at the Heinz's research farm (Stockton, CA). Tomato cvs Rutgers and Ohio 8245 were grown at the Purdue research farm and fruits were processed the same way as in California. Bostwick value for the juice processes from tomato cv Ohio 8245 was 15 ± 0.3 cm, whereas juice from tomato cv Rutgers fruits was too thin (Bostwick value >23 cm) to be measured by the standard Bostwick consistometer. B, Equal amounts of total RNAs from turning stages fruit of each line were size fractionated on an agarose gel, blotted to Hybond-N nylon membranes, and hybridized with α-³²P labeled vis1 cDNA as described in “Materials and Methods.” Also shown are the levels of 25S RNA. C, Ten micrograms of the genomic DNA from each tomato genotype was digested with EcoRI, separated on an agarose gel, blotted to a Hybond-N membrane, and hybridized with radiolabeled vis1 cDNA. The upper (12-kb) and lower (10-kb) hybridizing bands are designated as vis1-hta and vis1-lta alleles, respectively. D, Relationship between relative vis1 transcript accumulations and Bostwick values for different tomato genotypes. Relative transcript accumulation in fruits from different genotypes was quantified using InstantImager Electric Autoradiography (Packard Instrumental Company, Meriden, CO) and normalized for the amount of ribosomal RNA present in each sample.