Figure 4.

DNA polymorphism for vis1 is correlated with its transcript accumulation (A) in segregating progenies of tomato genotypes with vis1-hta or vis1-lta allele (B). A, Ripened fruits from each progeny obtained after selfing of tomato genotypes 70188 and 70274 (heterozygous for vis1-hta and vis1-lta alleles) were processed and Bostwick values were determined. Juice Bostwick values of vis1-hta and vis1-lta genotypes of lines 70274 and 70188 were significantly different at the 95% confidence level and correlated with vis1 expression. B, Identity of each segregating progeny, homozygous for vis1-hta or vis1-lta or heterozygous for the two alleles, was established by PCR of their individual genomic DNA followed by digestion with RsaI as described in “Materials and Methods.”