Figure 6.

³¹P NMR spectra of acid extracts of maize root tips. Samples of 2,000 excised root tips were filtered and frozen in liquid N₂ after 4-h incubation with 200 mm Glc (Control, top spectrum), or 48-h incubation with 100 mm 3-OMG (second spectrum from top), or 48-h incubation without substrate (bottom spectrum). The third spectrum from top was recorded after an addition of 0.4 mm G6P into the extract of 3-OMG-treated root tips. Acid extracts were prepared as described in “Materials and Methods” and pH was adjusted at 7.5. Spectra were acquired at 161.98 MHz with an FID resolution of 0.6 Hz, a 60° RF pulse (15 μs), and 2,048 transients repeated every 3.5 s. The exponential apodization was 0.5 Hz.