Table I.
Effect of 3-OMG on respiration, adenine nucleotide contents, ATP/ADP ratio, and AEC in maize root tips
| Condition | Respiration | ATP | ADP | AMP | Σ AdN | ATP/ADP | AEC |
|---|---|---|---|---|---|---|---|
| nmol O2 min−1 tip−1 | pmol tip−1 | ||||||
| Control | 3.45 ± 0.30 | 2,130 ± 382 | 360 ± 66 | 49 ± 26 | 2,540 ± 465 | 5.92 | 0.91 |
| Starvation | 0.70 ± 0.10 | 1,213 ± 273 | 225 ± 34 | 33 ± 17 | 1,471 ± 324 | 5.40 | 0.90 |
| 3-OMG (100 mm) | 0.85 ± 0.15 | 1,239 ± 141 | 216 ± 15 | 30 ± 8 | 1,485 ± 164 | 5.74 | 0.91 |
| 3-OMG (100 mm) + Pi (10 mm) | 0.78 ± 0.15 | 1,364 ± 367 | 270 ± 66 | 43 ± 30 | 1,677 ± 463 | 5.05 | 0.89 |
Except for control, defined as a 4-h incubation in 200 mm Glc, root tips were incubated for 48 h without carbon substrate or with 100 mm 3-OMG. When not mentioned, Pi concentration was 0.25 mm. AdNs were extracted and analyzed, and the values of Σ AdN and adenylate energy charge (AEC) were calculated as described in “Materials and Methods.” These data represent the mean of at least two (3-OMG and 3-OMG + Pi), four (starvation), and six (control) independent experiments.