Figure 3.

In situ detection of hSGK mRNA in intact kidneys and diabetic nephropathy. In normal kidney significant transcription of hSGK is found in a few mesangial cells and single epithelial cells of the distal tubule (A) and thick ascending limbs of Henle's loop (B, arrowheads). In diabetic kidneys (C–F), however, high levels of hSGK transcripts are found in numerous glomerular cells (C) and epithelial cells from the thick ascending limb of Henle's loop (D) as well as in clusters of interstitial cells of fibrotic areas (E). No labeling of cells was observed after hybridization with the α-³⁵S-labeled sense hSGK RNA probe (F). (×300.)