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Amino acid sequence alignment of CHIs. Conserved residues in type I CHIs (red), type II CHIs (blue), and both types of CHIs (green) are highlighted. The residues that compose the active site are indicated with asterisks. Arrows indicate the residues proposed to affect substrate preference between 6′-deoxychalcone and 6′-hydroxychalcone (Jez et al., 2000).
