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. 2003 Mar;131(3):952–962. doi: 10.1104/pp.011882

Figure 2.

Figure 2

Root pMtENOD11-gusA induction in membrane-separated coculture. a, b, d, f, and g, Ri T-DNA-transformed roots membrane-separated from G. rosea. c, e, and h, Control roots cultured with membrane separation but without fungus. a, GUS activity colocalizes with hyphal ramifications on other side of membrane (white arrowheads), bar = 0.2 cm. b, MtENOD11 induction occurs mainly in tertiary Ri T-DNA-transformed roots; c, only constitutive expression (see also e) is observed in control roots, bars = 2 cm. d, AM factor-induced GUS activity stretches from 0.1 cm behind the root cap as far as the zone of mature root hair growth, bar = 0.2 cm; e, in control roots, only constitutive expression is present in root caps and at the base of lateral roots, bar = 0.1 cm. f, Detail of pMtENOD11-gusA induction in epidermal cells including root hairs, bar = 50 μm. g and h, Seventy-five-micrometer-thick transverse sections of AM-inoculated (g) and control (h) roots, bars = 150 μm. i, Membrane-separated coculture of G. rosea with whole plants; j, control plants. i, pMtENOD11-gusA is induced in young lateral roots, bar = 0.3 cm; j, only background GUS activity is seen in vascular tissues in control roots, bar = 0.3 cm. k and l, pMtENOD11-gusA induction in membrane-separated coculture with other fungi. Induction occurs in Ri T-DNA-transformed roots cocultured with G. margarita (k), but not with Phy. medicaginis (l), bars = 0.6 cm. m, pMtENOD11-gusA induction in membrane-separated coculture of G. rosea and with Ri T-DNA-transformed roots derived from the M. truncatula Myc− mutant dmi2-2, bar = 0.7 cm.