Figure 4.

pMtENOD11-gusA induction in M. truncatula Nod⁻/Myc⁻ dmi mutants. Percentage of Ri T-DNA-transformed roots of M. truncatula wild-type and dmi mutants that show induction of pMtENOD11-gusA after 10 d of membrane-separated coculture with G. rosea (+AM) and without (−AM). Roots were counted in the “corridor” of dense fungal growth and hyphal ramifications. Data from three independent experiments were pooled and treated as subsets (blocks) of a single data set for statistical analysis. Means ± se labeled with a different letter (a and b) are significantly different according to analysis of variance followed by Tukey's test (P < 0.05, n = 7).