Figure 6.

Regulation of a Gmlbc3 promoter in V. hirsuta roots by CPP1. (A–D) Histochemical GUS staining of V. hirsuta nodule primordia, roots, and nodules. Different developmental stages of nodules from plants transformed with a Gmlbc3 promoter fused to gusA: (A and B) nodule primordia; (C) young nodules; and (D) a mature nodule. (E) Schematic presentation of the lbc3–GUS and lbc3–GUS 35S-CPP1 constructs. (F) Fluorometric measurement of GUS activity expressed from a Gmlbc3 promoter in the absence of CPP1 (lbc3–GUS) or in the presence of CPP1 (lbc3–GUS 35S-CPP1). The GUS activities determined in nodules and roots for each individual plant are indicated by diamonds on a logarithmic scale. Dashed lines indicate the mean values.