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. Author manuscript; available in PMC: 2006 Dec 1.
Published in final edited form as: Biochem Biophys Res Commun. 2006 Oct 2;350(4):834–841. doi: 10.1016/j.bbrc.2006.09.128

Fig. 4.

Fig. 4

[35S]Met labeling of products obtained from each of the constructs shown in Fig.1B. A. Synthetic RNA (60 ng) were used to program reticulocyte lysates (10 μl). Reactions were incubated for 45 min at 25ºC. Radiolabeled products were examined by SDS-PAGE and phosphorimaging. Controls included a reaction mixture programmed with a firefly LUC encoding mRNA lacking her-2 sequences (T7-LUC) and a reaction mixture to which no RNA was added (-RNA). B. Synthetic RNA (60 ng) were used to program wheat germ extracts (10 μl). Reactions were incubated for 30 min at 25ºC. In each panel, filled arrowheads correspond to polypeptide products (where present) arising from (i) initiation at AUG1 (lanes 6-7); (ii) initiation at AUG1 or possibly at a nearby CUG codon (lanes 1–4). The arrow corresponds to polypeptide products (where present) arising from possible initiation at AUG2 (lanes 1–4).