Table II.

White lupin ESTs derived from a cDNA library of proteoid roots at 7 and 10 DAE were hybridized against first strand cDNA of different tissues

EST Identification No.	GenBank Accession No. of EST	Accession No. of GenBank Hit	BLASTX E Value	Annotation	Possible Function	Ratio of Expressiona
						−P Proteoid Root/+P Normal Root			Normal Root	Leaf
						10 DAE	12 DAE	14 DAE	−P/+P	−P/+P
E22, E99, E372	CA410752, CA526338, CA526349	AAC16012.1	9E-76	Polyubiquitin	Here, serves as control	1 ± 0.2	0.9 ± 0.2	1.3 ± 0.3	1 ± 0.2	1 ± 0.2
E170, E292, E304	CA526339, CA411008, CA411018	Q9SJQ9	4E-47	Fru-1,6-bisP aldolase	Glycolysis	1.7 ± 0.7	2.2 ± 0.9	2.5 ± 1.4	0.9 ± 0.4	1.1 ± 0.3
E100, E111, E147, E440, E549, E625, E790	CA410825, CA410836, CA410872, CA411147, CA411156, CA411327, CA411489	Q9XG67	2E-62	NAD-dependent glyceraldehyde-3P dehydrogenase	Glycolysis	2.2 ± 0.8	2.2 ± 0.7	2.4 ± 0.8	1.3 ± 0.5	1.3 ± 0.4
E241	CA410959	O42908	1E-62	2,3-Bisphosphoglycerate mutase	Glycolysis	2.1 ± 0.2	3.7 ± 1.6	4.2 ± 1.2	1.3 ± 0.2	1.2 ± 0.7
E18	CA410748	CAB75428.1	1E-68	Enolase	Glycolysis	1.7 ± 0.1	2.4 ± 0.1	2.4 ± 0.3	1.4 ± 0.1	1.1 ± 0.5
E171, 447	CA410895, CA411154	AAL11502.1	5E-63	Malate dehydrogenase	Glycolysis	2.1 ± 0.2	2.4 ± 0.3	2.5 ± 0.4	1.5 ± 0.3	1.2 ± 0.8
E112, E423, E677	CA410837, CA411130, CA411379	AF520576	5E-27	Extensin-like protein	Cell wall structure protein	2.3 ± 0.6	1.6 ± 0.6	1.3 ± 0.7	1.4 ± 0.3	1.3 ± 0.4
E81, E757, E796	CA410808, CA411457, CA411495	BAB33421.1	2E-25	Putative senescence-associated protein	Unknown	2.1 ± 0.5	1.7 ± 0.8	2.2 ± 0.5	1.4 ± 0.4	0.8 ± 0.4
E183	CA410907	NP_198080.1	5E-17	Putative protein	Unknown	4.4 ± 0.2	3.8 ± 0.9	1.4 ± 0.8	0.4 ± 0.3	1.3 ± 0.3
E711	CA411412			No significant match	Unknown	1.2 ± 0.2	1.6 ± 1.1	5.1 ± 0.6	4.9 ± 0.1	0.8 ± 0.1

Listed are ESTs that displayed induced expression in −P proteoid roots, compared to +P normal roots.

^a Values represent averages from two independent nylon filter arrays.