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Medsa;cycA2;2 expression in non-symbiotic tissues. A, RT-PCR analysis of Medsa;cycA2;2 and the constitutive Msc27 gene expression in different tissues of alfalfa and in exponentially growing A2 cells. B, Detection of the CycA2 protein by western-blot analysis. For A and B, the roots, hypocotyls, and cotyledons were harvested from 1-week-old seedlings, whereas leaves and apical meristems were from 10-d-old plants.
