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Overexpression of cycA2;2 in transgenic M. truncatula plants does not correlate with increased level of the CycA2;2 protein. A, Southern-blot analysis of HindIII digested genomic DNAs hybridized with the hptII probe. B, Northern-blot analysis of cycA2;2 and c27 expression. Numbers below the blots show the relative signal intensities of cycA2;2 after normalization with the c27 signals. C, Western-blot analysis of the CycA2;2 protein with the stained protein membrane. C, Control.
