Skip to main content
. 2003 Mar;131(3):1220–1227. doi: 10.1104/pp.015206

Figure 3.

Figure 3

Localization of GA 7-oxidase (A, F, and K), seed-specific GA 20-oxidase (B, G, and L), recently cloned GA 20-oxidase from seedlings (C, H, and M), GA 3-oxidase (D, I, and N), and GA 2-oxidase (E, J, and O) mRNA in pumpkin seeds by in situ hybridization. All samples were sectioned longitudinally in a 90° angle with the plane of seeds at 50% (A, B, D, F, G, I, K, L, and N) or 36% (C, E, H, J, M, and O) mature index according to Graebe (1972). The sections were hybridized to either an antisense (A–J) or a sense (K–O) RNA probe of the entire respective cDNA (A, B, D, F, G, I, K, L, and N) or the predicted ORF (C, E, H, J, M, and O) that was labeled with digoxigenin. c, Cotyledon; gm, ground meristem; mp, micropylar end; p, protoderm; qc, quiescent center; ram, root apical meristem; sc, inner layer of the seed coat. Bar = 0.1 mm.