Figure 3.

Improved male organ development of F₁ transgenic maize plants treated with kinetin and TDZ. A, F₁ transgenic plants sprayed with a mixture of kinetin and TDZ (+) or surfactant alone (−). Advanced floral development was visible on chemically treated plants of lines S16802 and S16825 (+, Table I), whereas plants treated with surfactant only were devoid of floral development. B, Although florets appeared to develop on the transgenic plants after kinetin and TDZ applications, the pollen production was insufficient for pollination (++, Table I). Pollen viability stains were then performed using Alexander's solution (Alexander, 1969). The chemically restored florets produced viable (purple and symmetrical in shape) pollen (I) similar to wild type. Some of the transgenic plants failed to rescue completely resulting in nonviable (blue and asymmetrical in shape) pollen (II).