Table II.
Summary of unique sequences identified and no. of locus-specific primers used
DOP Primer | 3′ Enda | Unique/Total Sequencesb | Uniquec | Amplified in >2 Individualsd | Amplifiede |
---|---|---|---|---|---|
% | % | ||||
1 | aagcgatgt | 26/88 | 30 | 26 | 100 |
2 | aagcgatgact | 17/25 | 68 | 17 | 100 |
3 | atcgagctgac | 34/54 | 64 | 28 | 82 |
4 | accttggaacg | 32/55 | 58 | 29 | 91 |
5 | aagcgatgactg | 23/41 | 56 | 23 | 100 |
Total | 132/263 | 55 | 123 | 95 |
Composition of the arbitrary, specified regions of primers used in DOP-PCR.
No. of unique/total sequences encountered when sequencing cloned DOP-PCR products.
Percentage of DOP-PCR products displaying unique sequences in the cloned set.
No. of unique regions that were successfully amplified from genomic DNA of two or more discrete individuals using locus-specific primers designed according to the unique sequences identified from DOP-PCR products.
Percentage of unique sequences that could be recovered from two or more accessions of E. longifolia using locus-specific primers.