Figure 9.

LeGOLS-1 transcript accumulates in dehydration-stressed leaves and chilling-stressed seedlings. Mature leaves were detached from 18-d-old tomato plants and dehydrated to different moisture contents. The excised leaves were placed immediately in a plastic bag (0 min, 100% of initial fresh weight) or were allowed to lose water on the bench top for up to 1 h (20 min = 92%, 40 min = 88%, and 60 min = 88% of initial fresh weight) and then were sealed in plastic bags for 6 h before freezing and extraction of RNA. Plants were also sprayed once to run-off with 100 μm ABA and leaves harvested at 0, 6, 12, and 24 h after ABA application. Some plants were subjected to 4°C for 12, 24, and 48 h, and the leaves from some plants harvested, whereas other plants were moved to 25°C for an additional 48 h before the leaves were harvested. Some plants were placed at 37°C for 4, 8, and 12 h and leaves harvested at each time.