Skip to main content
. 2003 Mar;131(3):1431–1439. doi: 10.1104/pp.016915

Figure 6.

Figure 6

Comparison of folate accumulation, HPPK-DHPS mRNA and protein expression in light- and dark-grown pea seedlings. Pea seedlings were grown at 22°C in the light (with a photoperiod of 12 h) or in the dark for 7 d before sample harvesting and analysis. A, Folate content in the organs of light- and dark-grown pea seedlings. B, Relative abundance of the mRNA for HPPK-DHPS in green and etiolated leaves. The amount of mRNA coding HPPK-DHPS was measured by semiquantitative RT-PCR using total RNA isolated from green and etiolated leaves. The amount of mRNA detected in green leaves was used as a reference. C, Accumulation of the HPPK-DHPS protein in green and etiolated leaves. Soluble proteins (30 μg) were analyzed by western blot with antiserum to HPPK-DHPS. In A and B, values ± se are means of triplicate determinations for at least three independent experiments.