Figure 3.

Effects of GA₄ and IAA on the mRNA expression of CsAGP1. A, Total RNA was isolated from cucumber hypocotyls harvested after being treated with GA₄ (1 μg plant⁻¹) or IAA (10 μg plant⁻¹) for 1, 3, 6, and 12 h. B, Total RNA was isolated from the roots, hypocotyls, shoot apices, and cotyledons (harvested 2 h after being treated with GA₄ [1 μg plant⁻¹]). R, Roots; H, hypocotyls; A, shoot apices; C, cotyledons. Fifteen micrograms of total RNA was separated on 1% (w/v) agarose gel, and ethidium bromide staining of ribosomal RNAs (lower panels) was used to confirm the equivalent loading.