Table I.
Clone | bp | Putative Gene Function | Significance of Similarity | Accession No. | Experiment
1a
|
Experiment
2b
|
Experiment 3b
|
|||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
0 | 0.5 | 1 | 4 | − | + | − | + | |||||
Gros(RE)-DD1 | 291 | 28S rRNA gene | e−131 | AJ419662 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
Gros(RE)-DD2 | 335 | No similarity | – | AJ419663 | 0.19 | 0.32 | 0.43 | 0.88 | ndc | nd | nd | nd |
Gros(RE)-DD3 | 581 | Adrenal gland protein | 2e−15 | AJ419664 | 0 | 0.25 | 0.39 | 1.01 | nd | nd | nd | nd |
Gros(RE)-DD4 | 212 | P450-monooxygenase | e−07 | AJ419665 | 0.23 | 0.62 | 0.89 | 1.02 | nd | nd | nd | nd |
Gros(RE)-DD5 | 626 | NCO4 | 9e−16 | AJ419666 | 0 | 0 | 0.05 | 0.47 | 1.18 | 3.04 | 0.22 | 0.65 |
Gros(RE)-DD6 | 252 | 24S mt rRNA | 8e−19 | AJ419667 | 0.02 | 0.16 | 0.33 | 0.13 | nd | nd | nd | nd |
Gros(RE)-DD7 | 462 | 24S mt-rRNA | 6e−64 | AJ419668 | 0.35 | 0.9 | 0.98 | 0.53 | nd | nd | nd | nd |
Gros(RE)-60d | 435 | mt ATP/ADP translocase | e−14 | AJ419669 | nd | nd | nd | nd | 0.41 | 1.21 | 0.19 | 2.74 |
Gros(RE)-92d | 501 | Pyruvate carboxylase | e−51 | AJ419670 | nd | nd | nd | nd | 0.63 | 1.31 | 0.40 | 0.98 |
Size and putative functions of fragments isolated by differential display or by subtractive hybridization are shown as well as the percentage of identical amino acids over length of the alignment, the accession nos., and the relative levels of expression measured in different reverse northern-blot experiments.
Hours after addition of root exudates.
One hour of growth with water/methanol in the absence (−) or in the presence (+) of root exudates.
nd, Not determined.
Expressed sequence tags obtained from the subtractive library.