Table IV.
Compositions of total cell wall materials from endosperm tissues of T1 and T2 generation seeds from transgenic tobacco plants expressing fenugreek GMGT
| Transgenic or Control Line | Composition
|
Man/Gal Valueb | ||||
|---|---|---|---|---|---|---|
| Ara | Gal | Glc | Xyla | Man | ||
| mol % | ||||||
| T2 generation | ||||||
| FGT 1 | 17.0 | 17.0 | 4.4 | – | 61.7 | 3.5 |
| 16.1 | 15.1 | 4.4 | – | 64.3 | 4.1 | |
| FGT 6 | 20.1 | 16.0 | 4.2 | 2.8 | 56.8 | 3.6 |
| 19.8 | 16.1 | 4.0 | 2.4 | 57.7 | 3.6 | |
| FGT AA | 20.1 | 15.9 | 3.6 | 2.4 | 58.0 | 3.6 |
| 18.6 | 14.5 | 12.0 | 2.2 | 52.6 | 3.6 | |
| T1 generation | ||||||
| FGT 1 | 17.3 | 10.8 | 4.8 | 2.7 | 64.5 | 6.0 |
| 17.7 | 11.7 | 4.8 | – | 65.8 | 5.4 | |
| FGT 3 | 17.3 | 11.5 | 5.4 | – | 65.8 | 5.5 |
| 17.6 | 11.7 | 5.3 | – | 65.4 | 5.4 | |
| FGT 6 | 16.7 | 11.6 | 5.8 | – | 65.9 | 5.5 |
| 18.2 | 12.4 | 5.9 | 2.9 | 60.6 | 4.9 | |
| FGT O | 16.9 | 11.9 | 5.4 | – | 65.8 | 5.3 |
| 18.8 | 14.3 | 5.0 | – | 61.9 | 4.2 | |
| FGT L | 18.2 | 12.3 | 5.0 | – | 64.5 | 5.0 |
| 17.6 | 11.8 | 5.3 | – | 65.4 | 5.3 | |
| FGT AA | 18.6 | 14.9 | 3.8 | – | 62.6 | 4.0 |
| 18.9 | 15.2 | 4.8 | – | 61.0 | 3.8 | |
| Controls | ||||||
| CTR A | 18.4 | 10.2 | 3.5 | – | 67.9 | 6.4 |
| 19.5 | 10.0 | 4.0 | 2.4 | 64.2 | 6.4 | |
| CTR B | 18.4 | 9.2 | 4.5 | – | 67.8 | 7.1 |
| 17.8 | 9.3 | 5.4 | – | 67.5 | 7.0 | |
| CTR C | 19.0 | 10.2 | 3.8 | 2.4 | 64.5 | 6.3 |
| 17.7 | 10.2 | 3.7 | – | 68.3 | 6.4 | |
The T1 generation seeds include 25% azygous individuals without the transgene, whereas the T2 generation seeds include less than 3% of such individuals. Transgenics were independent lines, and control plants had been regenerated from tissue culture. Duplicate analyses are of independent batches of 50 seeds.
a
Where not listed, included in Man total.
b
Where independent figure for Xyl not given, 2.6 mol % Xyl assumed