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Uptake of sorbitol by transgenic yeast cells. RS453 cells were grown to the early logarithmic phase. Uptake of 0.5 mm [14C]sorbitol was measured at an external pH of 4.5. Three independent yeast clones transformed with PcSOT1 or PcSOT2 were tested (all with similar results) and compared with one clone expressing empty pDR196. The results are the means ± se of four replicates.
