Figure 2.

Amino acid sequences of PtFAD6 and PtFAD2 in comparison with the plastidial and microsomal homologs from rapeseed. For alignment, the ClustalX program (Thompson et al., 1997) was used (gap opening 12, gap extension 0.05). The conserved amino acids are on gray background. The three His boxes are indicated by subscripts, and the potential transmembrane domains are framed. The broken line, dots, and the arrow at the N terminus of PtFAD6 indicate an hydrophobic domain, hydroxylated fatty acids (Thr and Ser), and the N-terminal sequence of PtFAD6 fused to EGPF, respectively. The sequence data from P. tricornutum have been submitted to the DNA Data Bank of Japan/EMBL/GenBank sequence data bank (accession nos. AY165023 and AY165024 for PtFAD2 and PtFAD6, respectively).