Table I.
Substrate specificity of PtFAD2 expressed in Saccharomyces cerevisiae
| Substrate | Total Fatty Acids (Educt) | Product | Total Fatty Acids | Desaturation |
|---|---|---|---|---|
| % | % | % | ||
| a16:1Δ9 | 38.4 ± 1.6 | 16:2Δ9,12 | 6.6 ± 0.5 | 14.7 |
| b17:1Δ9 | 2.9 ± 0.2 | 17:2Δ9,12 | 0.8 ± 0.1 | 22.3 |
| a18:1Δ9 | 15.9 ± 1.1 | 18:2Δ9,12 | 16.1 ± 0.9 | 50.3 |
| 18:1Δ11 | 36.6 ± 2.2 | 18:2Δ11,14 | 0 | 0 |
| c20:1Δ11 | 9.5 ± 0.9 | 20:2Δ11,14 | 0.4 ± 0.1 | 4.1 |
| c22:1Δ13 | 2.0 ± 0.3 | 22:2Δ13,16 | 0 | 0 |
Yeast strain C13ABYS86 transformed with pYES2-PtFAD2 was grown for 48 h at 20°C in the presence or absence of different fatty acid substrates (500 μm or as indicated) in separate experiments. FAMEs from the whole cells were prepared and analyzed by GLC as indicated in “Materials and Methods.” Each value represents the percent of total fatty acids and is the mean ± sd from three independent experiments. Desaturation (percentage) was calculated as (product × 100)/(educt + product).
a
In the absence of exogenously fed fatty acid.
b
In the presence of 2 mm exogenously fed 17:0.
c
In the presence of 2 mm exogenously fed fatty acid.