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Immunoprecipitation of [α-32P]GTP-labeled monomeric G-proteins with anti-Rab 8 antibodies. A, KCl (750 mm)-extracted membrane proteins; B, 1% (w/v) Triton X-100-solubilized membrane proteins. Fractions were derived from epicotyls treated with ethylene (1 μL L−1, 20 min).
