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MAP kinase activity in pea epicotyls as affected by ethylene, MCP, and NBD. Pea seedlings were treated with ethylene (1 μL L−1, 20 min), MCP (100 nL L−1, 2 h), NBD (2,000 μL L−1, 2 h), or inhibitors of ethylene binding (as indicated above) followed by ethylene treatment. MAP kinase was assayed after precipitation with either anti-ERK1 (A) or anti-phospho-Tyr (B) antibodies by means of in-gel assays.
