Figure 2.

Northern-blot analysis of GmSMT1 expression in leaves (A) and tubers (B) from wild-type and transgenic GmSMT1 potato plants. Total RNA in young leaves and tubers was extracted from plants grown in a climate chamber or a greenhouse, respectively. Twenty micrograms of RNA was separated on formaldehyde gels, blotted onto a nylon filter, and hybridized with labeled DNA probes for GmSMT1 and rRNA (loading control). B, In tubers, a weak signal was detected for the clones 126 and 231 after prolonged exposure of the film.