Figure 3.

Southern-blot analysis of T-DNA-containing sequences in mmt mutant and wild-type Arabidopsis plants. Genomic DNA was extracted from plants shown by PCR to be homozygous for the mutant mmt allele or the wild-type MMT allele. The DNA was digested with the enzymes indicated. Blots were probed with the whole T-DNA region from the pD991 vector, containing the GUS and kanamycin resistance genes (Campisi et al., 1999). Genomic reconstructions were made with linearized pD991 DNA equivalent to one, two, and five copies per haploid genome; the results are consistent with insertion of a small number of copies of the T-DNA at a single locus in the mmt mutant. The positions of DNA size markers are indicated on the left.