Table III.

AdoMet and AdoHcy levels in leaves of wild-type and mmt mutant Arabidopsis

Genotype	AdoMet	AdoHcy	Methylation Ratio (AdoMet:AdoHcy)
	nmol g−1 fresh wt
Wild type	13.0 ± 0.4	1.37 ± 0.06	9.5 ± 0.4
mmt Mutant	14.9 ± 0.8*	1.09 ± 0.06**	13.8 ± 0.5***

AdoMet and AdoHcy were determined as their isoindole derivatives by fluorescence-HPLC using samples (0.15–0.2 g fresh wt) drawn from pooled rosette leaves harvested from five plants just before bolting. Data are means of four replicates ± se and have been corrected for recovery. Differences between wild type and mutant that are significant at P = 0.05, 0.01, or 0.001 are indicated by one, two, or three asterisks, respectively.