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. 2003 Apr;131(4):1834–1842. doi: 10.1104/pp.102.019174

Figure 1.

Figure 1

A, RT-PCR on cDNA from 2-d-old seedlings, leaf, root, and flower of untransformed wild-type Col0. Actin cDNA was amplified using primers ACT2S and ACT2A, PCK1 using PCK1-1001 and PCK1-1709, and PCK2 using PCK2-1032 and PCK2-1536. B, RT-PCR on cDNA from 2-d-old seedlings from untransformed wild-type Col0 and five 35S-PCK1 antisense lines: 64, 192, 122, 195, and 16. Endogenous (E) PCK1 cDNA only, was amplified using PCK1-72 and PCK1-573. Endogenous (E) and transgene (T) PCK1 cDNA was amplified using primers PCK1-1001 and PCK1-1709. C, PCK2 cDNA from 2-d-old seedlings was amplified using primers PCK2-1032 and PCK2-1536. PCR was carried out on undiluted (lanes 1), one-tenth (lanes 2), one-hundredth (lanes 3), and one-thousandth (lanes 4) dilutions of cDNA.