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. 2003 Apr;131(4):1868–1876. doi: 10.1104/pp.102.018572

Figure 4.

Figure 4

Identification by GC-MS of the acetate esters product formed in vitro by the product of RhAAT1. Bacterial lysates overexpressing RhAAT1 were incubated with geraniol and acetyl-CoA in assay buffer as described in “Materials and Methods.” The identification of the geranyl acetate product was done by matching with the retention time and the mass spectrum of authentic geranyl acetate, and by comparison with the computerized Wiley library. The Kovac index of geranyl acetate also corresponded with that of authentic standard. A, Lysates (derived from cells overexpressing RhAAT1) + geraniol + acetyl-CoA. B, Reaction buffer + geraniol + acetyl-CoA. C, Lysates derived from cells overexpressing RhAAT1 + acetyl-CoA. D, Lysates derived from cells overexpressing RhAAT1 + geraniol (no acetyl-CoA). E, Control lysates (derived from control E. coli BL21 [DE3] pLysS cells, not overexpressing RhAAT1) + geraniol + acetyl-CoA.