Table I.
Relative alchohol acetyltransferase activity of crude + 3 + 95 extracts derived from “Fragrant Cloud” rose petals (stages 4 and 5 combined) and of partially purified recombinant RhAAT gene product with selected alcohol substrates. Activity with geraniol was set as 100%. Means and ses of two replicates. The experiments were repeated three times with similar results.
| Substrate Testeda | Crude Extract | RhAAT1 |
|---|---|---|
| Monoterpene alcohols | ||
| Geraniol | 100 ± 15 | 100 ± 7.7 |
| Citronellol | 87 ± 8.8 | 60 ± 3.1 |
| Nerol | 35 ± 2.8 | 15.6 ± 1.6 |
| Linalool | 22 ± 0.3 | 3.6 ± 0.6 |
| Aromatic alcohols | ||
| 2-Phenylethyl alcohol | 144 ± 5.4 | 7.9 ± 0.5 |
| Benzyl alcohol | 35 ± 0.7 | 8.9 ± 0.5 |
| Aliphatic alcohols | ||
| cis-3-Hexene 1-ol | 237 ± 11 | 10.6 ± 1.2 |
| 1-Hexanol | 204 ± 21 | 13.9 ± 1 |
| 1-Octanol | 147 ± 7.7 | 34.8 ± 7 |
| 1-Butanol | 101 ± 11 | 4.2 ± 0.1 |
| Isoamyl alcohol | 97.4 ± 14 | 4.0 ± 2.0 |
| 1-Decanol | 50 ± 0.1 | 5.1 ± 0.2 |
| 3-Hexanol | 28.7 ± 1.2 | 4.5 ± 0.2 |
| Ethanol | 16.5 ± 1.2 | 2.4 ± 0.3 |
a
All substrates were tested at a conentration of 10 mm.