Table I.
Genes cloned by DDRT-PCR (DD/arbitrary primer no.) and subtractive hybridization with magnetic beads (SHMB) that exhibited nonsignificant (between 0.5 and 1.50) expression ratios in the microarray analysis and had similarity to genes in the databases
| Clone | Length | Method | Peak at h | Expression Ratio | Type | Accession No. | Sequence Similarity | E Value |
|---|---|---|---|---|---|---|---|---|
| bp | ||||||||
| DH017 | 1028 | SHMB | 24 | 1.33 | IIb | CA591807 | Matrix attachment regions-binding protein (MARBPF; AB059832) | 0.00 |
| DH108 | 453 | SHMB | 12 | 0.80 | III | CA591793 | PSII (NtPII10; X70088) | 0.00 |
| RE322 | 590 | DD/R5 | 24 | 1.25 | Ia | CA591771 | Transcription factor NtWRKY2 (AB020590) | 1 × 10–120 |
| DH123 | 371 | SHMB | 24 | 1.21 | IIa | CA591796 | Cytosolic glyceraldehyde-3-phosphophate dehydrogenase (GapC; M14419) | 2 × 10–94 |
| RE283 | 184 | DD/R5 | 12 | 0.63 | III | CA591769 | PSII oxygen-evolving complex, 23-kD polypeptide (X58910) | 1 × 10–73 |
| RD161 | 234 | DD/R4 | 12 | 1.47 | III | CA591756 | Actin gene Sfa 15B (X03076) | 1 × 10–47 |
| RB142 | 107 | DD/R2 | 24 | 1.13 | IIb | CA591717 | 25S ribosomal RNA gene (X13557) | 1 × 10–43 |
| RF064 | 161 | DD/R6 | 24 | 1.20 | IIb | CA591783 | 26S ribosomal RNA gene (AF479172) | 2 × 10–43 |
| RD131 | 190 | DD/R4 | 12 | 1.30 | III | CA591754 | Signal recognition particle 7S RNA (Z29099) | 3 × 10–35 |
| DH270 | 307 | SHMB | 6, 24 | 1.20, 1.23 | IV | CA591818 | Type 2 metallothionein-like protein (U35225) | 6 × 10–31 |
| RN021 | 389 | DD/R14 | 24 | 1.43 | Ia | CA591773 | Beta-alanine synthase (Y19104) | 3 × 10–30 |
| RN032 | 337 | DD/R14 | 12 | 0.54 | III | CA591774 | Polypeptide of PSII (X85038) | 1 × 10–17 |
| RC231 | 276 | DD/R3 | 12 | 1.23 | III | CA591748 | myb1 gene (AF248962) | 8 × 10–17 |
Genes are listed in order of decreasing E value from BLAST queries. Expression patterns are defined as: gradual (Type Ia) or abrupt (Type Ib) increases; initial decreases followed by either steady (Type IIa) or abrupt increases (Type IIb); an initial increase followed by a decrease (Type III); an increase, a decrease, and finally an increase (Type IV; both peaks are given if equal) as plants were continuously attacked by Manduca sexta larvae over 24 h. Down-regulated genes have the opposite patterns.