Table I.

Genes cloned by DDRT-PCR (DD/arbitrary primer no.) and subtractive hybridization with magnetic beads (SHMB) that exhibited nonsignificant (between 0.5 and 1.50) expression ratios in the microarray analysis and had similarity to genes in the databases

Clone	Length	Method	Peak at h	Expression Ratio	Type	Accession No.	Sequence Similarity	E Value
	bp
DH017	1028	SHMB	24	1.33	IIb	CA591807	Matrix attachment regions-binding protein (MARBPF; AB059832)	0.00
DH108	453	SHMB	12	0.80	III	CA591793	PSII (NtPII10; X70088)	0.00
RE322	590	DD/R5	24	1.25	Ia	CA591771	Transcription factor NtWRKY2 (AB020590)	1 × 10–120
DH123	371	SHMB	24	1.21	IIa	CA591796	Cytosolic glyceraldehyde-3-phosphophate dehydrogenase (GapC; M14419)	2 × 10–94
RE283	184	DD/R5	12	0.63	III	CA591769	PSII oxygen-evolving complex, 23-kD polypeptide (X58910)	1 × 10–73
RD161	234	DD/R4	12	1.47	III	CA591756	Actin gene Sfa 15B (X03076)	1 × 10–47
RB142	107	DD/R2	24	1.13	IIb	CA591717	25S ribosomal RNA gene (X13557)	1 × 10–43
RF064	161	DD/R6	24	1.20	IIb	CA591783	26S ribosomal RNA gene (AF479172)	2 × 10–43
RD131	190	DD/R4	12	1.30	III	CA591754	Signal recognition particle 7S RNA (Z29099)	3 × 10–35
DH270	307	SHMB	6, 24	1.20, 1.23	IV	CA591818	Type 2 metallothionein-like protein (U35225)	6 × 10–31
RN021	389	DD/R14	24	1.43	Ia	CA591773	Beta-alanine synthase (Y19104)	3 × 10–30
RN032	337	DD/R14	12	0.54	III	CA591774	Polypeptide of PSII (X85038)	1 × 10–17
RC231	276	DD/R3	12	1.23	III	CA591748	myb1 gene (AF248962)	8 × 10–17

Genes are listed in order of decreasing E value from BLAST queries. Expression patterns are defined as: gradual (Type Ia) or abrupt (Type Ib) increases; initial decreases followed by either steady (Type IIa) or abrupt increases (Type IIb); an initial increase followed by a decrease (Type III); an increase, a decrease, and finally an increase (Type IV; both peaks are given if equal) as plants were continuously attacked by Manduca sexta larvae over 24 h. Down-regulated genes have the opposite patterns.