Figure 7.
The accumulation of mRNA for GA 20-oxidase1 in transgenic plants that overexpress the potato knox gene, POTH1. A, Five micrograms of total RNA from the shoot tips of wild-type lines (designated 2, 9, and 10) and the overexpression lines, potato subsp. andigena 11, 15, and 18 were hybridized with a 1.2-kb fragment of the potato GA 20-oxidase1 cDNA, StGA20ox1 (Carrera et al., 1999). B, The membrane was stripped and reprobed with 18S wheat rRNA to ascertain equal loading and efficient transfer.